deseq2 normalized count data Search Results


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RStudio deseq2
(A) A phase-contrast image of untreated H9 ESCs (left) and 48 hour ACTIVIN A treated H9 ESCs (right) cultured as previously described (See ). (B) FACS analysis of TRA-1-60 antigen (sc-21750, Santa Cruz Biotech) of the two cell types described in panel A using BD Fortessa Analyzer. The y-axis indicates the number of cells and the x-axis indicates the FITC signal (TRA-1-60, untreated H9 ESCs: 93.1% +/- 0.92 positive vs ACTIVIN A: 51.2% +/- 1.1 positive; N = 2, p = 0.0076). The quantitation was limited to live cells by first removing debris and dead cells. (C) Immunostaining of the endodermal marker CXCR4 on untreated and 48 hour ACTIVIN A treated H9 ESCs. Images were acquired using identical exposure conditions for untreated and treated cells. Scale bars, 100 μm. (D) A volcano plot is shown, which presents the significance of each genes change in expression (p-adjusted) as a function of its fold change. The clear circles with black outlines, mostly found at the top of the plot, represent the change in gene expression after 6 hours. The filled gray circles indicate the change in gene expression after 48 hours. Genes from endoderm (filled red circles), mesoderm (filled blue squares), ectoderm (filled green triangles), and pluripotency-markers (filled black diamonds) are indicated within the plot. (E-H) The fold change in expression after 48 hours of ACTIVIN A treatment is shown for endoderm (E), mesoderm (F), ectoderm (G), and pluripotency markers (H). Significance testing was performed within <t>DESeq2</t> using the Benjamini/Hochberg correction to generate the adjusted p-value (p-adjusted), which represents a false discovery rate (FDR) of 10% .
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(A) A phase-contrast image of untreated H9 ESCs (left) and 48 hour ACTIVIN A treated H9 ESCs (right) cultured as previously described (See ). (B) FACS analysis of TRA-1-60 antigen (sc-21750, Santa Cruz Biotech) of the two cell types described in panel A using BD Fortessa Analyzer. The y-axis indicates the number of cells and the x-axis indicates the FITC signal (TRA-1-60, untreated H9 ESCs: 93.1% +/- 0.92 positive vs ACTIVIN A: 51.2% +/- 1.1 positive; N = 2, p = 0.0076). The quantitation was limited to live cells by first removing debris and dead cells. (C) Immunostaining of the endodermal marker CXCR4 on untreated and 48 hour ACTIVIN A treated H9 ESCs. Images were acquired using identical exposure conditions for untreated and treated cells. Scale bars, 100 μm. (D) A volcano plot is shown, which presents the significance of each genes change in expression (p-adjusted) as a function of its fold change. The clear circles with black outlines, mostly found at the top of the plot, represent the change in gene expression after 6 hours. The filled gray circles indicate the change in gene expression after 48 hours. Genes from endoderm (filled red circles), mesoderm (filled blue squares), ectoderm (filled green triangles), and pluripotency-markers (filled black diamonds) are indicated within the plot. (E-H) The fold change in expression after 48 hours of ACTIVIN A treatment is shown for endoderm (E), mesoderm (F), ectoderm (G), and pluripotency markers (H). Significance testing was performed within <t>DESeq2</t> using the Benjamini/Hochberg correction to generate the adjusted p-value (p-adjusted), which represents a false discovery rate (FDR) of 10% .
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(A) A phase-contrast image of untreated H9 ESCs (left) and 48 hour ACTIVIN A treated H9 ESCs (right) cultured as previously described (See ). (B) FACS analysis of TRA-1-60 antigen (sc-21750, Santa Cruz Biotech) of the two cell types described in panel A using BD Fortessa Analyzer. The y-axis indicates the number of cells and the x-axis indicates the FITC signal (TRA-1-60, untreated H9 ESCs: 93.1% +/- 0.92 positive vs ACTIVIN A: 51.2% +/- 1.1 positive; N = 2, p = 0.0076). The quantitation was limited to live cells by first removing debris and dead cells. (C) Immunostaining of the endodermal marker CXCR4 on untreated and 48 hour ACTIVIN A treated H9 ESCs. Images were acquired using identical exposure conditions for untreated and treated cells. Scale bars, 100 μm. (D) A volcano plot is shown, which presents the significance of each genes change in expression (p-adjusted) as a function of its fold change. The clear circles with black outlines, mostly found at the top of the plot, represent the change in gene expression after 6 hours. The filled gray circles indicate the change in gene expression after 48 hours. Genes from endoderm (filled red circles), mesoderm (filled blue squares), ectoderm (filled green triangles), and pluripotency-markers (filled black diamonds) are indicated within the plot. (E-H) The fold change in expression after 48 hours of ACTIVIN A treatment is shown for endoderm (E), mesoderm (F), ectoderm (G), and pluripotency markers (H). Significance testing was performed within <t>DESeq2</t> using the Benjamini/Hochberg correction to generate the adjusted p-value (p-adjusted), which represents a false discovery rate (FDR) of 10% .
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86
Partek deseq2
(A) A phase-contrast image of untreated H9 ESCs (left) and 48 hour ACTIVIN A treated H9 ESCs (right) cultured as previously described (See ). (B) FACS analysis of TRA-1-60 antigen (sc-21750, Santa Cruz Biotech) of the two cell types described in panel A using BD Fortessa Analyzer. The y-axis indicates the number of cells and the x-axis indicates the FITC signal (TRA-1-60, untreated H9 ESCs: 93.1% +/- 0.92 positive vs ACTIVIN A: 51.2% +/- 1.1 positive; N = 2, p = 0.0076). The quantitation was limited to live cells by first removing debris and dead cells. (C) Immunostaining of the endodermal marker CXCR4 on untreated and 48 hour ACTIVIN A treated H9 ESCs. Images were acquired using identical exposure conditions for untreated and treated cells. Scale bars, 100 μm. (D) A volcano plot is shown, which presents the significance of each genes change in expression (p-adjusted) as a function of its fold change. The clear circles with black outlines, mostly found at the top of the plot, represent the change in gene expression after 6 hours. The filled gray circles indicate the change in gene expression after 48 hours. Genes from endoderm (filled red circles), mesoderm (filled blue squares), ectoderm (filled green triangles), and pluripotency-markers (filled black diamonds) are indicated within the plot. (E-H) The fold change in expression after 48 hours of ACTIVIN A treatment is shown for endoderm (E), mesoderm (F), ectoderm (G), and pluripotency markers (H). Significance testing was performed within <t>DESeq2</t> using the Benjamini/Hochberg correction to generate the adjusted p-value (p-adjusted), which represents a false discovery rate (FDR) of 10% .
Deseq2, supplied by Partek, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Novogene deseq2 software package
(A) A phase-contrast image of untreated H9 ESCs (left) and 48 hour ACTIVIN A treated H9 ESCs (right) cultured as previously described (See ). (B) FACS analysis of TRA-1-60 antigen (sc-21750, Santa Cruz Biotech) of the two cell types described in panel A using BD Fortessa Analyzer. The y-axis indicates the number of cells and the x-axis indicates the FITC signal (TRA-1-60, untreated H9 ESCs: 93.1% +/- 0.92 positive vs ACTIVIN A: 51.2% +/- 1.1 positive; N = 2, p = 0.0076). The quantitation was limited to live cells by first removing debris and dead cells. (C) Immunostaining of the endodermal marker CXCR4 on untreated and 48 hour ACTIVIN A treated H9 ESCs. Images were acquired using identical exposure conditions for untreated and treated cells. Scale bars, 100 μm. (D) A volcano plot is shown, which presents the significance of each genes change in expression (p-adjusted) as a function of its fold change. The clear circles with black outlines, mostly found at the top of the plot, represent the change in gene expression after 6 hours. The filled gray circles indicate the change in gene expression after 48 hours. Genes from endoderm (filled red circles), mesoderm (filled blue squares), ectoderm (filled green triangles), and pluripotency-markers (filled black diamonds) are indicated within the plot. (E-H) The fold change in expression after 48 hours of ACTIVIN A treatment is shown for endoderm (E), mesoderm (F), ectoderm (G), and pluripotency markers (H). Significance testing was performed within <t>DESeq2</t> using the Benjamini/Hochberg correction to generate the adjusted p-value (p-adjusted), which represents a false discovery rate (FDR) of 10% .
Deseq2 Software Package, supplied by Novogene, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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deseq2 software package - by Bioz Stars, 2026-07
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Bluebee Inc lexogen quantseq de 1.2
(A) A phase-contrast image of untreated H9 ESCs (left) and 48 hour ACTIVIN A treated H9 ESCs (right) cultured as previously described (See ). (B) FACS analysis of TRA-1-60 antigen (sc-21750, Santa Cruz Biotech) of the two cell types described in panel A using BD Fortessa Analyzer. The y-axis indicates the number of cells and the x-axis indicates the FITC signal (TRA-1-60, untreated H9 ESCs: 93.1% +/- 0.92 positive vs ACTIVIN A: 51.2% +/- 1.1 positive; N = 2, p = 0.0076). The quantitation was limited to live cells by first removing debris and dead cells. (C) Immunostaining of the endodermal marker CXCR4 on untreated and 48 hour ACTIVIN A treated H9 ESCs. Images were acquired using identical exposure conditions for untreated and treated cells. Scale bars, 100 μm. (D) A volcano plot is shown, which presents the significance of each genes change in expression (p-adjusted) as a function of its fold change. The clear circles with black outlines, mostly found at the top of the plot, represent the change in gene expression after 6 hours. The filled gray circles indicate the change in gene expression after 48 hours. Genes from endoderm (filled red circles), mesoderm (filled blue squares), ectoderm (filled green triangles), and pluripotency-markers (filled black diamonds) are indicated within the plot. (E-H) The fold change in expression after 48 hours of ACTIVIN A treatment is shown for endoderm (E), mesoderm (F), ectoderm (G), and pluripotency markers (H). Significance testing was performed within <t>DESeq2</t> using the Benjamini/Hochberg correction to generate the adjusted p-value (p-adjusted), which represents a false discovery rate (FDR) of 10% .
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GraphPad Software Inc instat
(A) A phase-contrast image of untreated H9 ESCs (left) and 48 hour ACTIVIN A treated H9 ESCs (right) cultured as previously described (See ). (B) FACS analysis of TRA-1-60 antigen (sc-21750, Santa Cruz Biotech) of the two cell types described in panel A using BD Fortessa Analyzer. The y-axis indicates the number of cells and the x-axis indicates the FITC signal (TRA-1-60, untreated H9 ESCs: 93.1% +/- 0.92 positive vs ACTIVIN A: 51.2% +/- 1.1 positive; N = 2, p = 0.0076). The quantitation was limited to live cells by first removing debris and dead cells. (C) Immunostaining of the endodermal marker CXCR4 on untreated and 48 hour ACTIVIN A treated H9 ESCs. Images were acquired using identical exposure conditions for untreated and treated cells. Scale bars, 100 μm. (D) A volcano plot is shown, which presents the significance of each genes change in expression (p-adjusted) as a function of its fold change. The clear circles with black outlines, mostly found at the top of the plot, represent the change in gene expression after 6 hours. The filled gray circles indicate the change in gene expression after 48 hours. Genes from endoderm (filled red circles), mesoderm (filled blue squares), ectoderm (filled green triangles), and pluripotency-markers (filled black diamonds) are indicated within the plot. (E-H) The fold change in expression after 48 hours of ACTIVIN A treatment is shown for endoderm (E), mesoderm (F), ectoderm (G), and pluripotency markers (H). Significance testing was performed within <t>DESeq2</t> using the Benjamini/Hochberg correction to generate the adjusted p-value (p-adjusted), which represents a false discovery rate (FDR) of 10% .
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GraphPad Software Inc prism 7.0
(A) A phase-contrast image of untreated H9 ESCs (left) and 48 hour ACTIVIN A treated H9 ESCs (right) cultured as previously described (See ). (B) FACS analysis of TRA-1-60 antigen (sc-21750, Santa Cruz Biotech) of the two cell types described in panel A using BD Fortessa Analyzer. The y-axis indicates the number of cells and the x-axis indicates the FITC signal (TRA-1-60, untreated H9 ESCs: 93.1% +/- 0.92 positive vs ACTIVIN A: 51.2% +/- 1.1 positive; N = 2, p = 0.0076). The quantitation was limited to live cells by first removing debris and dead cells. (C) Immunostaining of the endodermal marker CXCR4 on untreated and 48 hour ACTIVIN A treated H9 ESCs. Images were acquired using identical exposure conditions for untreated and treated cells. Scale bars, 100 μm. (D) A volcano plot is shown, which presents the significance of each genes change in expression (p-adjusted) as a function of its fold change. The clear circles with black outlines, mostly found at the top of the plot, represent the change in gene expression after 6 hours. The filled gray circles indicate the change in gene expression after 48 hours. Genes from endoderm (filled red circles), mesoderm (filled blue squares), ectoderm (filled green triangles), and pluripotency-markers (filled black diamonds) are indicated within the plot. (E-H) The fold change in expression after 48 hours of ACTIVIN A treatment is shown for endoderm (E), mesoderm (F), ectoderm (G), and pluripotency markers (H). Significance testing was performed within <t>DESeq2</t> using the Benjamini/Hochberg correction to generate the adjusted p-value (p-adjusted), which represents a false discovery rate (FDR) of 10% .
Prism 7.0, supplied by GraphPad Software Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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RStudio r studio software
Volcano Plot generated using a <t>DESeq2</t> dataset, with base-10 log and base 2-fold change and P-value threshold of 0.05. In the plot the genes are colored if they pass thresholds for FDR and log fold change green indicates the upregulated genes and red colour indicates the downregulated genes, below the central line white colour indicates non-significant genes.
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RStudio rstudio version 4.3.2
Volcano Plot generated using a <t>DESeq2</t> dataset, with base-10 log and base 2-fold change and P-value threshold of 0.05. In the plot the genes are colored if they pass thresholds for FDR and log fold change green indicates the upregulated genes and red colour indicates the downregulated genes, below the central line white colour indicates non-significant genes.
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OmicSoft Corporation deseq2
Volcano Plot generated using a <t>DESeq2</t> dataset, with base-10 log and base 2-fold change and P-value threshold of 0.05. In the plot the genes are colored if they pass thresholds for FDR and log fold change green indicates the upregulated genes and red colour indicates the downregulated genes, below the central line white colour indicates non-significant genes.
Deseq2, supplied by OmicSoft Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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10X Genomics fgsea package v1 30 0 korotkevich
Volcano Plot generated using a <t>DESeq2</t> dataset, with base-10 log and base 2-fold change and P-value threshold of 0.05. In the plot the genes are colored if they pass thresholds for FDR and log fold change green indicates the upregulated genes and red colour indicates the downregulated genes, below the central line white colour indicates non-significant genes.
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Image Search Results


(A) A phase-contrast image of untreated H9 ESCs (left) and 48 hour ACTIVIN A treated H9 ESCs (right) cultured as previously described (See ). (B) FACS analysis of TRA-1-60 antigen (sc-21750, Santa Cruz Biotech) of the two cell types described in panel A using BD Fortessa Analyzer. The y-axis indicates the number of cells and the x-axis indicates the FITC signal (TRA-1-60, untreated H9 ESCs: 93.1% +/- 0.92 positive vs ACTIVIN A: 51.2% +/- 1.1 positive; N = 2, p = 0.0076). The quantitation was limited to live cells by first removing debris and dead cells. (C) Immunostaining of the endodermal marker CXCR4 on untreated and 48 hour ACTIVIN A treated H9 ESCs. Images were acquired using identical exposure conditions for untreated and treated cells. Scale bars, 100 μm. (D) A volcano plot is shown, which presents the significance of each genes change in expression (p-adjusted) as a function of its fold change. The clear circles with black outlines, mostly found at the top of the plot, represent the change in gene expression after 6 hours. The filled gray circles indicate the change in gene expression after 48 hours. Genes from endoderm (filled red circles), mesoderm (filled blue squares), ectoderm (filled green triangles), and pluripotency-markers (filled black diamonds) are indicated within the plot. (E-H) The fold change in expression after 48 hours of ACTIVIN A treatment is shown for endoderm (E), mesoderm (F), ectoderm (G), and pluripotency markers (H). Significance testing was performed within DESeq2 using the Benjamini/Hochberg correction to generate the adjusted p-value (p-adjusted), which represents a false discovery rate (FDR) of 10% .

Journal: PLoS ONE

Article Title: The Regulation of rRNA Gene Transcription during Directed Differentiation of Human Embryonic Stem Cells

doi: 10.1371/journal.pone.0157276

Figure Lengend Snippet: (A) A phase-contrast image of untreated H9 ESCs (left) and 48 hour ACTIVIN A treated H9 ESCs (right) cultured as previously described (See ). (B) FACS analysis of TRA-1-60 antigen (sc-21750, Santa Cruz Biotech) of the two cell types described in panel A using BD Fortessa Analyzer. The y-axis indicates the number of cells and the x-axis indicates the FITC signal (TRA-1-60, untreated H9 ESCs: 93.1% +/- 0.92 positive vs ACTIVIN A: 51.2% +/- 1.1 positive; N = 2, p = 0.0076). The quantitation was limited to live cells by first removing debris and dead cells. (C) Immunostaining of the endodermal marker CXCR4 on untreated and 48 hour ACTIVIN A treated H9 ESCs. Images were acquired using identical exposure conditions for untreated and treated cells. Scale bars, 100 μm. (D) A volcano plot is shown, which presents the significance of each genes change in expression (p-adjusted) as a function of its fold change. The clear circles with black outlines, mostly found at the top of the plot, represent the change in gene expression after 6 hours. The filled gray circles indicate the change in gene expression after 48 hours. Genes from endoderm (filled red circles), mesoderm (filled blue squares), ectoderm (filled green triangles), and pluripotency-markers (filled black diamonds) are indicated within the plot. (E-H) The fold change in expression after 48 hours of ACTIVIN A treatment is shown for endoderm (E), mesoderm (F), ectoderm (G), and pluripotency markers (H). Significance testing was performed within DESeq2 using the Benjamini/Hochberg correction to generate the adjusted p-value (p-adjusted), which represents a false discovery rate (FDR) of 10% .

Article Snippet: This was input into DESeq2 within R studio for differential expression analysis, using a Benjamini/Hochberg corrected p-value cut-off of 0.1 (p-adjusted) [ ].

Techniques: Cell Culture, Quantitation Assay, Immunostaining, Marker, Expressing, Gene Expression

Volcano Plot generated using a DESeq2 dataset, with base-10 log and base 2-fold change and P-value threshold of 0.05. In the plot the genes are colored if they pass thresholds for FDR and log fold change green indicates the upregulated genes and red colour indicates the downregulated genes, below the central line white colour indicates non-significant genes.

Journal: Saudi Journal of Biological Sciences

Article Title: Identification of key biomarkers and associated pathways of pancreatic cancer using integrated transcriptomic and gene network analysis

doi: 10.1016/j.sjbs.2023.103819

Figure Lengend Snippet: Volcano Plot generated using a DESeq2 dataset, with base-10 log and base 2-fold change and P-value threshold of 0.05. In the plot the genes are colored if they pass thresholds for FDR and log fold change green indicates the upregulated genes and red colour indicates the downregulated genes, below the central line white colour indicates non-significant genes.

Article Snippet: DESeq2 is a Bioconductor package used in R studio software for analyzing RNA-Seq data for Differential Expression analysis which uses negative binomial generalized linear models to identify statistically significant DEGs ( )( ).

Techniques: Generated